New Microscope Interface Enables High-Quality Imaging and Spectroscopy with a Single System
IsoPlane spectrograph shown MicroSpec interface and inverted microscope.
February 12, 2013
February 12, 2013 — Princeton Instruments (Trenton, New Jersey, USA) is pleased to announce the availability of
a new microscope interface designed for exclusive use with the company’s recently introduced aberration-free IsoPlane® imaging
spectrograph. The Princeton Instruments MicroSpec® microscope interface allows researchers to easily acquire quality images and
spectra of their samples with one pre-engineered system.
The MicroSpec attaches to a UDP port on select Olympus, Nikon, and Zeiss inverted microscopes. The interface is not compatible
with any other spectrographs, nor with upright microscopes. It supports all Princeton Instruments cameras with a spectroscopy
mount and without an internal shutter. The MicroSpec interface provides outstanding imaging of up to 12 x 12 mm field of view.
Whereas traditional Czerny-Turner (CT) spectrographs suffer from aberrations that degrade imaging performance and spectral quality,
particularly at the edges of the focal plane, the IsoPlane SCT 320 spectrograph provides superior imaging and spectra across the
focal plane. Thanks to having zero astigmatism and negligible spherical aberration and coma, the image produced at zero order at
the focal plane of the IsoPlane is of high quality. The lack of aberrations also means more photons hit fewer pixels,
increasing the signal-to-noise ratio of spectra.
“When coupled to a microscope, the IsoPlane can resolve up to 25 line pairs per mm, the Nyquist limit of CCD cameras with 20 micron
pixels,” states Ed Gooding, Spectroscopy Product Manager at Princeton Instruments. “The IsoPlane can be used from the vacuum UV to
the mid-IR, with software-controlled scanning and an interchangeable triple grating turret. It also supports IntelliCal ™,
our state-of-the-art wavelength and intensity calibration package. No other spectrometer on the market offers the IsoPlane’s
combination of flexibility, resolution, and extraordinary signal-to-noise ratio”.
Applications of the new MicroSpec-IsoPlane system include fluorescence, Raman, photoluminescence,
single-molecule spectroscopy, quantum dots, live-cell imaging, and carbon nanotube SWIR imaging and sum frequency generation.
About Princeton Instruments
Princeton Instruments designs and manufactures high-performance CCD, ICCD, EMCCD,
and InGaAs cameras; optical spectrometers; and optics-based solutions for the scientific research,
industrial imaging, and OEM communities. We take pride in partnering with our customers to solve their most
challenging problems in unique, innovative ways. Princeton Instruments is a registered ISO 9001:2008 company.
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