Flash photolysis spectrometer helps with light harvesting research
LP920 Laser Flash Photolysis spectrometer
December 5, 2012
Edinburgh Instruments, Photonics Division, Livingston, UK, 5th December 2012.
Researchers at the University of Connecticut have been using the
LP920 flash photolysis spectrometer
from Edinburgh Instruments
as part of their research into plant light harvesting complexes1. A paper has been published2 with collaborators at Kwansei Gakuin
University examining the efficiency of variants of the Peridinin-Chlorophyll a-Protein (PCP) complex in providing photoprotection
from singlet oxygen formation. PCP’s highly effective protective capacity against these photodynamic reactions is extremely
important since singlet oxygen can directly provoke cellular damage in plants by rapidly oxidizing cellular components.
The LP920 was used to record transient triplet-minus-singlet absorption spectra of chlorophyll a with different peridinin
molecular analogs in polar and non-polar solvents, allowing the dynamics of the reactions to be investigated. Although the
results showed that the spectral bands are shifted depending on the molecular analog used, the dynamics of triplet state decay
remain very similar for each analog meaning that there is no marked difference between them in terms of their ability to protect
against singlet oxygen formation.
The LP920 is a computer-controlled, fully automated flash photolysis spectrometer, equipped with a large sample chamber to house
a variety of sample holders. Excitation pulses at 660 -670 nm for these experiments were provided using an Nd:YAG-pumped laser
light source. A pulsed, highintensity 450 W Xenon lamp was used for the transient absorption spectral measurements
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